The conditioning (CP), incubation (IP), and aeration (AP) phases of the disinfection cycle can be distinguished by following the rH curve. (a) Representative graphs of relative humidity (rH) and surface temperature (T) during airborne disinfection in a biosafety cabinet. Microbiological efficacy testing of aerosolized peroxyacetic acid and hydrogen peroxide (aPAA-HP). Thus, we conclude that this method is an excellent choice for control of the current SARS-CoV-2 pandemic.īacillus subtilis Geobacillus stearothermophilus Indiana vesiculovirus (VSIV) Mycobacterium senegalense airborne disinfection dry fog murine norovirus (MNV) peroxyacetic acid room disinfection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For these reasons and due to the relative simplicity of the procedure, it is an ideal disinfection method for hospital wards, ambulances, public conveyances, and indoor community areas. In addition, the low concentration of dispersed disinfectant, particularly for enveloped viral pathogens such as SARS-CoV-2, entails high material compatibility. We show that dry fogging an aerosolized mixture of peroxyacetic acid and hydrogen peroxide (aPAA-HP) is highly microbicidal, efficient, fast, robust, environmentally neutral, and a suitable airborne disinfection method. IMPORTANCE Airborne disinfection is not only of crucial importance for the safe operation of laboratories and animal rooms where infectious agents are handled but also can be used in public health emergencies such as the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Furthermore, CSC are artificial bioindicators with lower resistance and thus should not be used for validating airborne disinfection when microorganisms other than viruses have to be inactivated. Overall, we conclude that dry fogging a mixture of aPAA-HP is highly effective against a broad range of microorganisms as well as material compatible with relevant concentrations. Furthermore, we found striking differences in inactivation efficacies against some of the tested microorganisms. Relevant process parameters such as temperature and relative humidity can be wirelessly monitored. Our results demonstrate that aPAA-HP is a highly efficient procedure for airborne room disinfection. We then used the QCT methodology to determine relevant process parameters to develop and validate effective disinfection protocols (≥4-log 10 reduction) in various large and complex facilities. We used quantitative carrier testing (QCT) procedures to compare the sensitivity of CSC with that of surrogates for nonenveloped and enveloped viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), mycobacteria, and spores, to an aerosolized mixture of peroxyacetic acid and hydrogen peroxide (aPAA-HP). Commercial spore carriers (CSC) coated with 10 6 spores of Geobacillus stearothermophilus are often used to assess the efficacy of disinfection. Airborne disinfection of high-containment facilities before maintenance or between animal studies is crucial.
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